4SCIENTIFIC REVIEW Role of Auxilin and Heat Shock Protein 70kDa in Clathrin Uncoating
نویسنده
چکیده
An interaction between auxilin and heat shock protein 70kDa (Hsc70) was initially discovered in 1995. There exists a large amount of data supporting the basis for their interaction in vitro and their function in clathrin uncoating in vivo. This review examines the key experiments in elucidating this interaction and introduces a third protein that may connect constriction or fission with hsc70/auxilin mediated clathrin uncoating.
منابع مشابه
Inhibition of hsc70-catalysed clathrin uncoating by HSJ1 proteins.
The uncoating of clathrin-coated vesicles can be mediated in vitro by the 'uncoating ATPase' that has been identified as the constitutive 70 kDa heat shock protein (hsp70), hsc70. It is now established that the activity of hsp70 proteins can be regulated by another family of molecular chaperones, the DnaJ family. In this study, we have investigated the effects of DnaJ-like proteins (the human n...
متن کاملThe auxilin-like phosphoprotein Swa2p is required for clathrin function in yeast
BACKGROUND In eukaryotic cells, clathrin-coated vesicles transport specific cargo from the plasma membrane and trans-Golgi network to the endosomal system. Removal of the clathrin coat in vitro requires the uncoating ATPase Hsc70 and its DnaJ cofactor auxilin. To date, a requirement for auxilin and Hsc70 in clathrin function in vivo has not been demonstrated. RESULTS The Saccharomyces cerevis...
متن کاملClathrin uncoating: Auxilin comes to life
The DnaJ protein auxilin has been extensively studied in vitro as a cofactor for uncoating clathrin-coated vesicles by the chaperone Hsc70. Recent studies provide the first evidence that auxilin plays this role in vivo, and work on a new mammalian auxilin suggests the protein may have more complex cellular functions.
متن کاملA motif in the clathrin heavy chain required for the Hsc70/auxilin uncoating reaction.
The 70-kDa heat-shock cognate protein (Hsc70) chaperone is an ATP-dependent "disassembly enzyme" for many subcellular structures, including clathrin-coated vesicles where it functions as an uncoating ATPase. Hsc70, and its cochaperone auxilin together catalyze coat disassembly. Like other members of the Hsp70 chaperone family, it is thought that ATP-bound Hsc70 recognizes the clathrin triskelio...
متن کاملUncoating of Clathrin-Coated Vesicles in Presynaptic Terminals Roles for Hsc70 and Auxilin
We have examined the roles of Hsc70 and auxilin in the uncoating of clathrin-coated vesicles (CCVs) during neuronal endocytosis. We identified two peptides that inhibit the ability of Hsc70 and auxilin to uncoat CCVs in vitro. When injected into nerve terminals, these peptides inhibited both synaptic transmission and CCV uncoating. Mutation of a conserved HPD motif within the J domain of auxili...
متن کامل